Considerations when combining 3D cell culture with higher throughput screening

Considerations when combining 3D cell culture with high throughput screening Accurately predicting in vivo drug toxicity and efficacy is of paramount significance in the course of pre-clinical testing phases - if a candidate drug fails through human trials, it represents a substantial waste of each time and money. Enhanced preclinical screening of candidate molecules making use of cell culture models that accurately represent in vivo effects under in vitro circumstances can assist to avoid this. In this weblog, we explore some examples of how novel 3D approaches are enhancing drug discovery in cancer investigation.

Similar cells, distinct response

While 3D Cell Culture market share is becoming far better optimised for high throughput screening (HTS) programmes, it is generally routinely used for smaller-scale validation experiments. Dr Olivier Pardo of Imperial College, London, highlighted the added benefits of this strategy at a current SMi Cell Culture conference. His data illustrate how tumour cells grown under 2D situations respond differently to the exact same concentration of an anti-tumour drug when when compared with 3D cell cultures, as cells inside the 3D environment are more resistant for the compound. Dr Pardo’s research also recommend that the unique levels of oxygen availability in 2D vs 3D, at the same time because the extent of cell-cell speak to, can influence therapeutic response to a drug compound.



Upscaling challenges

3D cell culture systems may be used to create data which is much more biologically relevant. Nevertheless, combining this strategy with HTS workflows can nevertheless be challenging. Thankfully, innovative corporations are meeting this challenge head-on, seeing it as an chance to capture market share by creating novel procedures that facilitate HTS assays applying 3D cell culture on an industrial scale.

In recent years a number of automation-friendly plate and assay formats have become commercially available to culture massive numbers of cells in 3D, like ultra low attachment and hanging drop plates for tumour spheroids and soft agar systems for cell colonies.

Novel approaches to render 3D culture amenable to HTS

A variety of new technological advancements is allowing researchers to benefit from the advantages offered by 3d cell cultures technologies and global markets, without having compromising on throughput speed. From automated imagers capable of effectively capturing the detail provided by 3D cultures, for the improvement of fully synthetic, self-assembling nanofibres that support cells developing in higher throughput microplates, this thrilling field continues to redefine how compounds are screened and tested prior to clinical trials. To find out far more, speak to our specialist group to talk about your needs or arrange a demo of acumen Cellista.

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